NCI-H520-EGFP cell line

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NCI-H520-EGFP Order

NCI-H520-EGFP cell line
Catalog#
YC-C017-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium-D
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NCI-H520-EGFP cell line
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Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
NCI-H520-EGFP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Accession
CVCL_C8X0(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H520		 STR Info (Cell bank)
Cell Line:NCI-H520 [H520]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 10111011
D7S820 812812
D16S539 813813
VWA 18191819
TH01 10101010
AMEL XXXX
TPOX 8888
CSF1PO 10101010
D12S391 2121
FGA 2222
D2S1338 1823
D21S11 3030
D18S51 1717
D8S1179 141617.0
D3S1358 1616
D6S1043 1218
PENTAE 514
D19S433 1314
PENTAD 1313
D1S1656 1416.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the NCI-H520 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NCI-H520-EGFP cell line that can maintain long-term, stable and high expression.
NCI-H520-EGFP cell line
NCI-H520-EGFP
Catalog#
YC-C017-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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